Abstract Background Heterotrichous canon imageclass mf227dw ciliates are common members of microeukaryote communities which play important roles in both the transfer of material and the flow of energy in aquatic food webs.This group has been known for over two centuries due to their large body size and cosmopolitan distribution.Nevertheless, species identification and phylogenetic relationships of heterotrichs remain challenging due to the lack of accurate morphological information and insufficient molecular data.Results The morphology and phylogeny of two heterotrichous ciliates, namely Gruberia foissneri spec.
nov.and Linostomella vorticella (Ehrenberg, 1833) Aescht in Foissner et al., 1999, were studied using rigorous methods (living morphology, stained preparations, and small subunit rDNA sequence data).Gruberia foissneri spec.
nov.is morphologically very similar to G.uninucleata Kahl, 1932, however, it can be distinguished from the latter by having more ciliary rows (about 32 vs.about 20) and macronuclear shape (sausage-shaped vs.
ellipsoid).Based on a combination of previous and present studies, an improved diagnosis of L.vorticella is supplied and several taxonomic anomalies are clarified.In addition, phylogenetic analyses based on SSU rDNA sequence data support the generic assignment of these two species.
Conclusions Modern ciliate taxonomy should be performed by means of detailed living observation, stained preparations and molecular information.For those species that have been reported in previous studies, it is necessary to provide as much useful usc trojans snapback hat information as possible using state-of-the-art methods in order to resolve taxonomic anomalies.